通过消减克隆鉴定大鼠实验性食管肿瘤中改变的细胞角蛋白亚类。

Identification of cytokeratin subspecies altered in rat experimental esophageal tumors by subtractive cloning.

作者信息

Wang D Y, Xiang Y Y, Tanaka M, Shen Q, Sugimura H

机构信息

First Department of Pathology, Hamamatsu University School of Medicine, Japan.

出版信息

Cancer Lett. 1996 Nov 12;108(1):119-27. doi: 10.1016/s0304-3835(96)04403-5.

DOI:10.1016/s0304-3835(96)04403-5

PMID:8950218

Abstract

By using the subtractive hybridization method, two complementary DNA clones differently expressed in rat normal esophageal epithelium and squamous cell carcinoma induced by administration of precursors of N-nitrososarcosine ethyl ester were isolated. A rat homologue of the human 50-kDa type I cytokeratin 14 was cloned for the first time and shown to be expressed preferentially in squamous cell papillomas and carcinomas, whereas it was weakly expressed or absent in normal squamous epithelial cells and in hyperplastic lesions. A rat homologue of the mouse 57-kDa type II cytokeratin showed strong expression in both normal and tumor tissues. These results are well consistent with the reported alteration of keratin subspecies in human esophageal cancers, therefore, encouraging us to use this experimental system as a model for human esophageal carcinogenesis.

摘要

通过采用消减杂交法，分离出了两个在大鼠正常食管上皮和由N-亚硝基肌氨酸乙酯前体诱导产生的鳞状细胞癌中差异表达的互补DNA克隆。首次克隆出了人类50-kDa I型细胞角蛋白14的大鼠同源物，结果显示其在鳞状细胞乳头状瘤和癌中优先表达，而在正常鳞状上皮细胞和增生性病变中表达较弱或缺失。小鼠57-kDa II型细胞角蛋白的大鼠同源物在正常组织和肿瘤组织中均有强表达。这些结果与报道的人类食管癌中角蛋白亚型的改变高度一致，因此，促使我们将这个实验系统用作人类食管癌发生的模型。