Inhibitory guanine nucleotide regulatory protein alpha-subunits in brain of spontaneously hypertensive rats.

Previously we have reported a decreased GABAB receptor expression in brain of spontaneously hepertensive rats (SHR). Moreover, decreased receptor numbers were accompanied by depressed stimulation of cAMP formation. We have now quantified Gi alpha protein by immunoblotting in cerebral membranes from 11-week-old normotensive Wistar-Kyoto rats (WKY) and hepertensive SHR. The levels of the alpha-subunit in the two sets of membranes were similar. In conclusion, the reduced response in adenylate cyclase activity to agents that function via the Gi protein, e.g., GABAB receptor agonists, in SHR brain is not a consequence of altered level of Gi alpha protein.