Young C Y, Seay T, Hogen K, Charlesworth M C, Roche P C, Klee G G, Tindall D J
Department of Urology, Medical School, Mayo Clinic/Foundation, Rochester, Minnesota 55905, USA.
Prostate Suppl. 1996;7:17-24.
Although prostate-specific antigen (PSA) has aided significantly in the diagnosis of prostate cancer, more sensitive and accurate assays are needed. Presently, we are developing a sensitive immunoassay for hK2 protein for the detection of prostate cancer.
Polyclonal and monoclonal antibodies specific for hK2 were produced and used for Western blot analysis and immunohistochemistry for detection of hK2 protein in serum and human tissues. The reverse-transcriptase polymerase chain reaction (RT-PCR) was utilized to detect hK2 mRNA from patient blood samples.
Western blot analysis demonstrated that the antibodies used are monospecific for hK2. Immunohistochemistry showed that hK2 is expressed only in prostatic epithelia. An RT-PCR study showed that hK2 mRNA would be a useful candidate for early detection of prostatic micrometastasis.
Monospecific antibodies for hK2 have been developed for detecting hK2 protein. Our studies indicate that hK2 may be a useful marker for prostate cancer.
尽管前列腺特异性抗原（PSA）在前列腺癌的诊断中发挥了重要作用，但仍需要更敏感、准确的检测方法。目前，我们正在开发一种用于检测前列腺癌的hK2蛋白敏感免疫测定法。
制备了针对hK2的多克隆和单克隆抗体，并用于蛋白质印迹分析和免疫组织化学，以检测血清和人体组织中的hK2蛋白。利用逆转录聚合酶链反应（RT-PCR）检测患者血样中的hK2 mRNA。
蛋白质印迹分析表明，所使用的抗体对hK2具有单特异性。免疫组织化学显示，hK2仅在前列腺上皮中表达。一项RT-PCR研究表明，hK2 mRNA可能有助于早期检测前列腺微转移。
已开发出用于检测hK2蛋白的hK2单特异性抗体。我们的研究表明，hK2可能是一种有用的前列腺癌标志物。
