Baghian A, Kousoulas K, Truax R, Storz J
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine Louisiana State University, Baton Rouge 70803-8416, USA.
Am J Vet Res. 1996 Dec;57(12):1720-5.
To analyze the antigenic cross reactivity of various proteins of strains of Chlamydia pecorum, C psitaci, and C trachomatis. SAMPLES AND PROCEDURES: Strains FC-Stra and LW-613 of C pecorum, strains B577, Fitz-9, and 68C of C psitaci, and strain LGV-2 of C trachomatis were studied. Strains of C pecorum were propagated in Georgia bovine kidney cells, and other chlamydial strains were propagated in L cells or Georgia bovine kidney cells. Partially purified chlamydial elementary bodies propagated in RAG cells, a BALB/c cell line cloned from a renal adenocarcinoma of BALB/c mice, were used to immunize BALB/c mice for production of monoclonal antibodies. Rabbits were inoculated with yolk sack-propagated, purified elementary bodies to produced polyclonal antisera. The reaction of monoclonal antibodies and polyclonal antisera with chlamydial proteins was analyzed by use of immunoblot techniques.
Two monoclonal antibodies reacted with a 90-kd protein of C psittaci and a 94-kd protein of C pecorum strains. One monoclonal antibody reacted strongly with a 67-kd protein of C pecorum and strain B577 of C psittaci, but weakly with proteins of strains 6BC and LGV-2. Another monoclonal antibody reacted with a 46-kd protein of 2 C pecorum strains and of strain B577 of psittaci, but not with those of strains 6BC and LGV-2. Two monoclonal antibodies reacted with a 20-kd protein of C pecorum and a 22-kd protein of C psittaci and LGV-2 strains. Polyclonal antisera reacted similarly with the proteins identified by monoclonal antibodies in the various chlamydial strains.
Reactions of several monoclonal antibodies with chlamydial antigens indicated that 67- and 46-kd proteins contain genus- and species-specific epitopes, respectively; a 94-kd protein of C pecorum is homologous to a 90-kd protein of C psittaci and C trachomatis strains; and a 20-kd protein of C pecorum corresponds to a 22-kd protein of C psittaci and C trachomatis.
分析鹦鹉热衣原体、沙眼衣原体和猪衣原体各菌株多种蛋白质的抗原交叉反应性。
研究了猪衣原体的FC-Stra和LW-613菌株、鹦鹉热衣原体的B577、Fitz-9和68C菌株以及沙眼衣原体的LGV-2菌株。猪衣原体菌株在佐治亚牛肾细胞中繁殖，其他衣原体菌株在L细胞或佐治亚牛肾细胞中繁殖。在RAG细胞（一种从BALB/c小鼠肾腺癌克隆的BALB/c细胞系）中繁殖的部分纯化衣原体原体用于免疫BALB/c小鼠以产生单克隆抗体。用卵黄囊繁殖的纯化原体接种兔子以产生多克隆抗血清。通过免疫印迹技术分析单克隆抗体和多克隆抗血清与衣原体蛋白的反应。
两种单克隆抗体与鹦鹉热衣原体的一种90-kd蛋白和猪衣原体菌株的一种94-kd蛋白发生反应。一种单克隆抗体与猪衣原体的一种67-kd蛋白和鹦鹉热衣原体的B577菌株强烈反应，但与6BC和LGV-2菌株的蛋白反应较弱。另一种单克隆抗体与两种猪衣原体菌株和鹦鹉热衣原体B577菌株的一种46-kd蛋白发生反应，但与6BC和LGV-2菌株的蛋白不反应。两种单克隆抗体与猪衣原体的一种20-kd蛋白以及鹦鹉热衣原体和LGV-2菌株的一种22-kd蛋白发生反应。多克隆抗血清与各种衣原体菌株中由单克隆抗体鉴定的蛋白反应相似。
几种单克隆抗体与衣原体抗原的反应表明，67-kd和46-kd蛋白分别含有属特异性和种特异性表位；猪衣原体的一种94-kd蛋白与鹦鹉热衣原体和沙眼衣原体菌株的一种90-kd蛋白同源；猪衣原体的一种20-kd蛋白对应于鹦鹉热衣原体和沙眼衣原体的一种22-kd蛋白。
