Dubey J P, Andrews C D, Lind P, Kwok O C, Thulliez P, Lunney J K
USDA, Agricultural Research Service, Beltsville, MD 20705-2350, USA.
Am J Vet Res. 1996 Dec;57(12):1733-7.
To follow antibody responses measured by various serologic tests in pigs orally inoculated with low (< or = 10 oocysts) numbers of Toxoplasma gondii oocysts.
24, 2- to 3-month-old pigs.
Pigs (n = 42) were inoculated orally with 10 (14 pigs) or 1 (28 pigs) infective oocysts, and 6 pigs served as uninoculated controls. Blood (serum) samples were obtained at 1- to 3-week intervals until euthanasia. At necropsy, the brain, heart, and tongue of pigs were bioassayed in mice and cats for isolation of T gondii. Modified agglutination test (MAT), using whole, fixed tachyzoites and mercaptoethanol; latex agglutination test (LAT); indirect hemagglutination test (IHAT); Sabin-Feldman dye test (DT); and ELISA were used to evaluate serologic responses to T gondii.
T gondii was isolated from tissues of 13 of 14 pigs each fed 10 oocysts, 17 of 28 pigs each fed 1 oocyst, and 0 of 6 control pigs. 29 of 30 T gondii-infected pigs developed antibodies when measured by MAT, DT, and ELISA; the 1 seronegative-infected pig had been fed 10 oocysts and was euthanatized 69 days after inoculation. LAT detected antibodies in 26 of 30 T gondii-infected pigs. IHAT detected antibodies in 11 T gondii-infected pigs.
MAT, DT, and ELISA were more sensitive serologic assays than LAT and IHAT for detecting antibodies induced by low numbers of T gondii in pigs.
追踪经口接种少量（≤10个卵囊）刚地弓形虫卵囊的猪通过各种血清学检测测得的抗体反应。
24头2至3月龄的猪。
给42头猪经口接种10个（14头猪）或1个（28头猪）感染性卵囊，6头猪作为未接种对照。每隔1至3周采集血（血清）样，直至实施安乐死。尸检时，取猪的脑、心脏和舌在小鼠和猫身上进行生物测定以分离刚地弓形虫。采用完整、固定的速殖子和巯基乙醇的改良凝集试验（MAT）；乳胶凝集试验（LAT）；间接血凝试验（IHAT）；Sabin-Feldman染色试验（DT）；以及酶联免疫吸附测定（ELISA）来评估对刚地弓形虫的血清学反应。
在每头投喂10个卵囊的14头猪中，有13头猪的组织分离出刚地弓形虫；在每头投喂1个卵囊的28头猪中，有17头猪的组织分离出刚地弓形虫；6头对照猪中均未分离出。通过MAT、DT和ELISA检测，30头感染刚地弓形虫的猪中有29头产生了抗体；1头血清学阴性的感染猪投喂了10个卵囊，接种后69天实施安乐死。LAT在30头感染刚地弓形虫的猪中检测到26头有抗体。IHAT在11头感染刚地弓形虫的猪中检测到抗体。
对于检测猪因少量刚地弓形虫诱导产生的抗体，MAT、DT和ELISA比LAT和IHAT是更敏感的血清学检测方法。
