转化生长因子-β2增加牛视网膜色素上皮细胞中α-平滑肌肌动蛋白的表达。
TGF-beta 2 increases alpha-smooth muscle actin expression in bovine retinal pigment epithelial cells.
作者信息
Kurosaka D, Muraki Y, Inoue M, Katsura H
机构信息
Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Japan.
出版信息
Curr Eye Res. 1996 Nov;15(11):1144-7. doi: 10.3109/02713689608995147.
PURPOSE
To examine whether transforming growth factor-beta 2 (TGF-beta 2) induces the expression of alpha-smooth muscle actin (SMA), a biochemical marker of myofibroblasts, in cultured bovine retinal pigment epithelial (RPE) cells.
METHODS
Bovine RPE cells were cultured in F-12 nutrient mixture supplemented with 5% fetal bovine serum (FBS), with or without TGF-beta 2 (0.01-10 ng/ml) for 6 days. During this culture period, cells did not reach a confluence. Alpha-SMA was detected immunocytochemically with a mouse monoclonal antibody, and the ratio of the number of alpha-SMA positive cells to the total number of cells was calculated.
RESULTS
About 10% of the cells in control cultures with only FBS were positive for alpha-SMA. TGF-beta 2 increased the ratio of positive cells dose-dependently (p < 0.0001), while a neutralizing antibody against TGF-beta 2 blocked this effect.
CONCLUSIONS
TGF-beta 2 induced expression of alpha-SMA in bovine RPE cells.
目的
研究转化生长因子-β2（TGF-β2）是否能诱导培养的牛视网膜色素上皮（RPE）细胞中α-平滑肌肌动蛋白（SMA）的表达，α-平滑肌肌动蛋白是肌成纤维细胞的一种生化标志物。
方法
将牛RPE细胞培养于添加5%胎牛血清（FBS）的F-12营养混合物中，分别添加或不添加TGF-β2（0.01 - 10 ng/ml），培养6天。在此培养期间，细胞未达到汇合状态。用小鼠单克隆抗体通过免疫细胞化学法检测α-SMA，并计算α-SMA阳性细胞数与细胞总数的比例。
结果
仅含FBS的对照培养物中约10%的细胞α-SMA呈阳性。TGF-β2使阳性细胞比例呈剂量依赖性增加（p < 0.0001），而抗TGF-β2中和抗体可阻断此效应。
结论
TGF-β2诱导牛RPE细胞中α-SMA的表达。
Zotero 插件