Fisker S, Orskov H
Medical Department M (Diabetes and Endocrinology, University Hospital of Aarhus, Denmark.
Horm Res. 1996;46(4-5):183-7. doi: 10.1159/000185020.
Growth hormone (GH) in human serum has been measured for more than 30 years and during this period it has been used diagnostically in growth disturbances and GH disorders. The first GH determinations were by radioimmunoassays using polyclonal antibodies. Due to the low specificity of these assays and the variable molecular appearance of GH, higher levels were obtained than with later assays employing two highly specific monoclonal antibodies. Because of this variability between assays, empiric GH cutoff levels had to be developed in different laboratories. However, with the growing need for more sophisticated diagnosis, especially in adulthood GH deficiency, knowledge of factors influencing the individual GH assays is required. Since GH deficiency in adulthood is now treated with GH substitution therapy, the relevance of factors influencing GH estimates has become increasingly evident, because no specific symptoms exist in adult GH deficiency.
人体血清中的生长激素（GH）已被检测了30多年，在此期间，它被用于生长障碍和GH紊乱的诊断。最初的GH测定是通过使用多克隆抗体的放射免疫测定法进行的。由于这些测定法的特异性较低以及GH分子外观的变化，与后来使用两种高度特异性单克隆抗体的测定法相比，得到的GH水平更高。由于不同测定法之间存在这种差异，不同实验室不得不制定经验性的GH临界值水平。然而，随着对更精确诊断的需求不断增加，尤其是在成人GH缺乏症方面，需要了解影响个体GH测定的因素。由于现在成人GH缺乏症采用GH替代疗法进行治疗，影响GH估计值的因素的相关性变得越来越明显，因为成人GH缺乏症没有特定症状。
