Wagar E A
Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90095-1732, USA.
J Clin Lab Anal. 1996;10(6):331-4. doi: 10.1002/(SICI)1098-2825(1996)10:6<331::AID-JCLA4>3.0.CO;2-9.
The purpose of this article is to describe specific applications of molecular diagnostics that are currently identifying suspected or unidentified microbial pathogens. The techniques reviewed include (i) the use of specific primers and PCR to identify new microbes, (ii) PCR amplification of conserved 16S rRNA sequence with subsequent identification of specific internal sequence from the candidate bacterial pathogen, and (iii) an exciting new modification of PCR called RDA or "reverse PCR" that can identify unique infectious agents in diseased tissue. The field will continue to expand rapidly and, it is hoped, contribute to a better understanding of the microbial environment with which humans coexist. Also, molecular techniques will eventually be applied in the demonstration of pathogenesis by the various newly identified microbial pathogens.
本文的目的是描述分子诊断的具体应用，这些应用目前正在识别疑似或未识别的微生物病原体。所综述的技术包括：（i）使用特异性引物和聚合酶链反应（PCR）来识别新的微生物；（ii）对保守的16S核糖体RNA（rRNA）序列进行PCR扩增，随后从候选细菌病原体中鉴定特定的内部序列；以及（iii）一种名为RDA或“反向PCR”的令人兴奋的PCR新改良方法，它可以识别患病组织中的独特感染因子。该领域将继续迅速扩展，并且有望有助于更好地理解人类共存的微生物环境。此外，分子技术最终将应用于各种新鉴定的微生物病原体的致病机制研究。
