Johnston R N, Halton D W, Anderson P A, Johnston C F, Shaw C
Comparative Neuroendocrinology Research Group, Schools of Clinical Medicine and Biology/Biochemistry, Queen's University of Belfast, Northern Ireland.
J Comp Neurol. 1996 Dec 9;376(2):214-22. doi: 10.1002/(SICI)1096-9861(19961209)376:2<214::AID-CNE4>3.0.CO;2-6.
The organisation of the nervous system of Bdelloura candida (Tricladida, Maricola, and Bdellouridae) was studied by immunocytochemistry, by using an antiserum raised to the authentic B. candida FMRFamide-related peptide (FaRP), GYIRFamide. Immunostaining was intense and abundant throughout both the central and peripheral nervous systems, being localised to the brain, the longitudinal nerve cords and their transverse and lateral connections, the pharyngeal plexus, the extensive sub-epidermal and sub-muscular plexuses, and elements of the reproductive apparatus. Compared to an earlier anatomical investigation of this species, and also to the neuroanatomy of other triclad turbellarians, the pattern of GYIRFamide-immunoreactivity reveals differences in the following aspects: the shape and structure of the brain, the distribution of longitudinal nerve cords and their relationships with the peripheral nervous system, the structure and distribution of the lateral nerves and the transverse connectives between the longitudinal nerve cords, organisation of the pharyngeal nervous system, and innervation of the eyespots and epidermal sensory structures. Although this study focuses on a descriptive account of the neuroanatomy of Bdelloura candida, by using anti-GYIRFamide as a neuronal marker, the possible functions of the native peptide are also discussed. The quality and reproducibility of the immunostaining obtained during this work highlights the effectiveness of the GYIRFamide antiserum in the neuroanatomical study of flatworms, and also the suitability of B. candida as a model species in studies of the turbellarian nervous system.
通过免疫细胞化学方法，利用针对真白蛭（Bdelloura candida）FMRF酰胺相关肽（FaRP）GYIRF酰胺制备的抗血清，对真白蛭（三肠目、海生涡虫类、白蛭科）的神经系统组织进行了研究。免疫染色在中枢神经系统和外周神经系统中均强烈且丰富，定位于脑、纵神经索及其横向和侧向连接、咽神经丛、广泛的表皮下和肌下神经丛以及生殖器官的组成部分。与该物种早期的解剖学研究以及其他三肠目涡虫的神经解剖学相比，GYIRF酰胺免疫反应模式在以下方面显示出差异：脑的形状和结构、纵神经索的分布及其与外周神经系统的关系、侧神经的结构和分布以及纵神经索之间的横向连接、咽神经系统的组织以及眼点和表皮感觉结构的神经支配。尽管本研究重点描述了真白蛭的神经解剖学，但通过使用抗GYIRF酰胺作为神经元标记，还讨论了天然肽的可能功能。在这项工作中获得的免疫染色的质量和可重复性突出了GYIRF酰胺抗血清在扁虫神经解剖学研究中的有效性，以及真白蛭作为涡虫神经系统研究模型物种的适用性。
