Costa M, Brookes S J, Steele P A, Gibbins I, Burcher E, Kandiah C J
Department of Human Physiology, Flinders University of South Australia, Adelaide, Australia.
Neuroscience. 1996 Dec;75(3):949-67. doi: 10.1016/0306-4522(96)00275-8.
A strategy has been developed to identify and quantify the different neurochemical populations of myenteric neurons in the guinea-pig ileum using double-labelling fluorescence immunohistochemistry of whole-mount preparations. First, six histochemical markers were used to identify exclusive, non-overlapping populations of nerve cell bodies. They included immunoreactivity for the calcium binding proteins calbindin and calretinin, the neuropeptides vasoactive intestinal polypeptide, substance P and somatostatin, and the amine, 5-hydroxytryptamine. The sizes of these populations of neurons were established directly or indirectly in double-labelling experiments using a marker for all nerve cell bodies. Each of these exclusive populations was further subdivided into classes by other markers, including immunoreactivity for enkephalins and neurofilament protein triplet. The size of each class was then established directly or by calculation. These distinct, neurochemically-identified classes were related to other published work on the histochemistry, electrophysiology and retrograde labelling of enteric neurons and to the simple Dogiel morphological classification. A classification scheme, consistent with previous studies, is proposed. It includes 14 distinct classes of myenteric neurons and accounts for nearly all neurons in the myenteric plexus of the guinea-pig ileum.
已开发出一种策略，通过对整装标本进行双标荧光免疫组织化学，来识别和量化豚鼠回肠中肌间神经元的不同神经化学群体。首先，使用六种组织化学标记物来识别神经细胞体的排他性、非重叠群体。它们包括钙结合蛋白钙结合蛋白和钙视网膜蛋白、神经肽血管活性肠肽、P物质和生长抑素以及胺5-羟色胺的免疫反应性。在使用所有神经细胞体标记物的双标实验中，直接或间接地确定了这些神经元群体的大小。这些排他性群体中的每一个都通过其他标记物进一步细分为不同类别，包括脑啡肽和神经丝蛋白三联体的免疫反应性。然后直接或通过计算确定每个类别的大小。这些通过神经化学鉴定的不同类别与先前发表的关于肠神经元组织化学、电生理学和逆行标记的其他研究以及简单的多吉尔形态学分类相关。提出了一种与先前研究一致的分类方案。它包括14种不同类别的肌间神经元，几乎涵盖了豚鼠回肠肌间神经丛中的所有神经元。
