Matsumoto I, Davidson M, Otsuki M, Wilce P A
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Pharmacol Biochem Behav. 1996 Nov;55(3):371-8. doi: 10.1016/s0091-3057(96)00106-2.
The involvement of kainate (KA)-sensitive regions in ethanol withdrawal behaviors was investigated in male Wistar rats given three intraperitoneal (IP) injections of KA (12 mg/kg) or saline each followed by recovery at 4 degrees C for 5 h and room temperature for 3 days and a final KA or saline injection at room temperature. Some animals received MK-801 (1 mg/kg, IP) 30 min after each injection and one group received saline only. The saline/saline, saline/MK-801, and KA/MK-801 groups displayed typical ethanol withdrawal behaviors 8-12 h after ethanol withdrawal. These behaviors were attenuated in the KA/saline group. Audiogenic seizures could be induced in all treatment groups 12 h after withdrawal. There was severe neuronal degeneration in the hippocampal CA region and the piriform cortex of the KA/saline-treated animals that was reduced by MK-801 treatment. The inferior colliculus remained intact. These results suggest that the N-methyl-D-aspartate receptor mediates KA-induced damage in limbic structures and that these regions may play an important role in typical, but not audiogenically induced ethanol-withdrawal behaviors.
在雄性Wistar大鼠中研究了对红藻氨酸（KA）敏感区域在乙醇戒断行为中的作用。给大鼠腹腔注射（IP）三次KA（12mg/kg）或生理盐水，每次注射后先在4℃恢复5小时，然后在室温下恢复3天，最后在室温下再注射一次KA或生理盐水。一些动物在每次注射后30分钟接受MK-801（1mg/kg，腹腔注射），一组只接受生理盐水。生理盐水/生理盐水组、生理盐水/MK-801组和KA/MK-801组在乙醇戒断后8 - 12小时表现出典型的乙醇戒断行为。这些行为在KA/生理盐水组中减弱。戒断12小时后，所有治疗组均可诱发听源性惊厥。KA/生理盐水处理的动物海马CA区和梨状皮质存在严重的神经元变性，而MK-801处理可减轻这种变性。下丘保持完整。这些结果表明，N-甲基-D-天冬氨酸受体介导KA诱导的边缘结构损伤，并且这些区域可能在典型的而非听源性诱导的乙醇戒断行为中起重要作用。
