de Groote K, Velghe G, Stals H K, Baes M I, Declercq P E
Faculty of Pharmaceutical Sciences, Department of Clinical Chemistry, Leuven, Belgium.
Cell Biochem Funct. 1996 Dec;14(4):277-81. doi: 10.1002/cbf.691.
After incubating isolated rat hepatocytes with [1-14 C]palmitic acid, CoA and ATP (+MgCl2), a significant amount of [1-14 C]palmitoyl-CoA was found in the incubation medium. There was no correlation between its rate of synthesis and the degree of intactness of the cells. The results indicate that there is a long-chain fatty acyl-CoA synthetase active on the external surface of the hepatocyte plasma membrane. The activity of this enzyme was negligible in primary cultures of rat hepatocytes, suggesting that the exofacial long-chain acyl-CoA synthetase is an artifact of the collagenase perfusion technique used to prepare the hepatocytes.
用[1-¹⁴C]棕榈酸、辅酶A和ATP（+MgCl₂）孵育分离的大鼠肝细胞后，在孵育培养基中发现了大量的[1-¹⁴C]棕榈酰辅酶A。其合成速率与细胞完整程度之间没有相关性。结果表明，在肝细胞质膜外表面存在一种活性长链脂肪酰辅酶A合成酶。该酶在大鼠肝细胞原代培养物中的活性可忽略不计，这表明外表面长链酰基辅酶A合成酶是用于制备肝细胞的胶原酶灌注技术的人为产物。
