食蟹猴疟原虫红细胞外期在恒河猴肝细胞中的体外培养
In-vitro cultivation of exoerythrocytic stages of Plasmodium cynomolgi in hepatocytes of Macaca radiata.
作者信息
Kamboj K K, Puri S K, Katiyar J C, Dutta G P
机构信息
Division of Parasitology, Microbiology, Central Drug Research Institute, Lucknow, India.
出版信息
Indian J Malariol. 1996 Jun;33(2):67-73.
PMID:8952170
Abstract
Hepatocytes from bonet monkey (Macaca radiata) obtained by perfusion of a liver biopsy were infected in-vitro with Plasmodium cynomolgi bastianellii sporozoites raised in Anopheles stephensi. The development of exoerythrocytic (EE) stages was seen under phase contrast microscope and by Giemsa staining. Multinucleated EE-stages were seen in the cultured hepatocytes on day 7-8 post-sporozoite inoculation.
摘要
通过肝脏活检灌注获得的食蟹猴（Macaca radiata）肝细胞，在体外被感染了在斯氏按蚊体内培育的食蟹猴疟原虫巴斯蒂安内利亚种子孢子。在相差显微镜下和通过吉姆萨染色观察到了红细胞外期（EE）的发育情况。在接种子孢子后第7 - 8天，在培养的肝细胞中观察到了多核的红细胞外期。
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