Rezzoug H, Barberi-Heyob M, Merlin J L, Bolotine L, Lignon D, Guillemin F
Unité de recherche en thérapie photodynamique, centre Alexis-Vautrin, URA CNRS, D0821, Vandoeuvre-lès-Nancy, France.
Bull Cancer. 1996 Oct;83(10):816-22.
Meso-tetra (hydroxyphenyl) chlorin (mTHPC) was evaluated in vitro in HT29 human colon adenocarcinoma cell line and compared with hematoporphyrin derivative (HpD). The incorporation kinetics, evaluated by flow cytometry showed that mTHPC and HpD cellular uptake was related to the incubation time until 12 hours, then a plateau appeared. Cytotoxicity assays were performed using MTT test after photoirradiation at 650 nm for mTHPC and 630 nm for HpD. The photodynamic activity of both photosensitizers was influenced by serum protein of the culture medium and time interval between irradiation and photocytotoxicity test with a maximal activity 24 and 48 hrs after the photoirradiation. In optimized experimental conditions i.e. 2% serum protein- containing culture medium, 24 hr-incubation period, cytotoxicity measured 48 hrs after exposure, 10 J/cm2 light fluence, mTHPC appeared approximately 20-fold more active than HpD with IC50 values of 0.2 micrograms/mL and 4.2 micrograms/mL, respectively.
在HT29人结肠腺癌细胞系中对中位四（羟苯基）氯卟啉（mTHPC）进行了体外评估，并与血卟啉衍生物（HpD）进行了比较。通过流式细胞术评估的摄取动力学表明，mTHPC和HpD的细胞摄取与直至12小时的孵育时间相关，然后出现平台期。在分别对mTHPC进行650nm和对HpD进行630nm光照射后，使用MTT试验进行细胞毒性测定。两种光敏剂的光动力活性受培养基血清蛋白以及照射与光细胞毒性试验之间的时间间隔影响，在光照射后24小时和48小时具有最大活性。在优化的实验条件下，即含2%血清蛋白的培养基、24小时孵育期、暴露后48小时测量细胞毒性、10 J/cm2的光通量，mTHPC的活性比HpD高约20倍，IC50值分别为0.2微克/毫升和4.2微克/毫升。
