Ezawa K, Minato K, Dobashi K
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Biomed Pharmacother. 1996;50(6-7):283-9. doi: 10.1016/0753-3322(96)84827-x.
We studied the direct anti-tumor effects of ubenimex on five human lung cancer cell lines; ABC-1, RERF-LC-OK, RERF-LC-MS (adenocarcinoma) and SQ-5, EBC-1 (squamous cell carcinoma). Ubenimex dose-dependently inhibited the growth of these cancer cell lines except RERF-LC-MS. The results indicated that lung squamous cell carcinoma cell lines were more sensitive to ubenimex than lung adenocarcinoma cell lines. Coincidentally, histological observation by Hematoxylin eosine (HE) staining revealed that ubenimex induced nuclear condensation and apoptic body in the cancer cell lines. Immunohistochemical study showed ubenimex-treated cells expressed LeY antigen which is a useful phenotypic marker predictive of apoptosis. The induction of DNA fragmentation was also observed in the ubenimex treated cancer cell lines by ELISA. We conclude that ubenimex exhibits its direct anti-tumor effect against non-small-cell lung cancer cell lines, more effectively against squamous carcinoma cell lines, through the induction of apoptosis.
我们研究了乌苯美司对五种人肺癌细胞系的直接抗肿瘤作用；ABC-1、RERF-LC-OK、RERF-LC-MS（腺癌）以及SQ-5、EBC-1（鳞状细胞癌）。除RERF-LC-MS外，乌苯美司呈剂量依赖性地抑制这些癌细胞系的生长。结果表明，肺鳞状细胞癌细胞系对乌苯美司比肺腺癌细胞系更敏感。巧合的是，苏木精伊红（HE）染色的组织学观察显示，乌苯美司在癌细胞系中诱导了核浓缩和凋亡小体。免疫组织化学研究表明，经乌苯美司处理的细胞表达LeY抗原，这是一种预测凋亡的有用表型标志物。通过酶联免疫吸附测定（ELISA）在经乌苯美司处理的癌细胞系中也观察到了DNA片段化。我们得出结论，乌苯美司通过诱导凋亡对非小细胞肺癌细胞系表现出直接抗肿瘤作用，对鳞状癌细胞系的作用更有效。
