DnaK3是蓝藻聚球藻属PCC7942的三种DnaK蛋白之一，可在类囊体膜中进行定量检测。
DnaK3, one of the three DnaK proteins of cyanobacterium Synechococcus sp. PCC7942, is quantitatively detected in the thylakoid membrane.
作者信息
Nimura K, Yoshikawa H, Takahashi H
机构信息
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
出版信息
Biochem Biophys Res Commun. 1996 Dec 4;229(1):334-40. doi: 10.1006/bbrc.1996.1802.
Subcellular localization of three DnaK proteins of cyanobacterium Synechococcus sp. PCC7942 was determined. DnaK1 and DnaK2 proteins were detected mainly in the cytosolic fraction. On the other hand, the DnaK3 protein occurred in large amounts in the thylakoid membrane fraction. Furthermore, DnaK3 was found to be located on the surface of the thylakoid membrane on the cytosol side. Subcellular localization of chimeric and truncated DnaK3 proteins was also determined, and it was suggested that the region a.a. 381 to a.a. 597 of DnaK3 protein, which is considered to correspond to the peptide-binding domain, was required for the association with the thylakoid membrane.
确定了蓝藻聚球藻属PCC7942三种DnaK蛋白的亚细胞定位。DnaK1和DnaK2蛋白主要在胞质部分被检测到。另一方面，DnaK3蛋白大量存在于类囊体膜部分。此外，发现DnaK3位于类囊体膜胞质侧表面。还确定了嵌合和截短的DnaK3蛋白的亚细胞定位，结果表明，DnaK3蛋白中被认为对应于肽结合结构域的381位氨基酸至597位氨基酸区域是与类囊体膜结合所必需的。
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