聚球藻Synechococcus elongatus PCC 7942中多个dnaK基因的表达分析。
Expression analysis of multiple dnaK genes in the cyanobacterium Synechococcus elongatus PCC 7942.
作者信息
Sato Masumi, Nimura-Matsune Kaori, Watanabe Satoru, Chibazakura Taku, Yoshikawa Hirofumi
机构信息
Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
出版信息
J Bacteriol. 2007 May;189(10):3751-8. doi: 10.1128/JB.01722-06. Epub 2007 Mar 9.
Abstract
We analyzed the stress responses of three dnaK homologues (dnaK1, dnaK2, and dnaK3) in the cyanobacterium Synechococcus elongatus PCC 7942. A reporter assay showed that under stress conditions the expression of only the dnaK2 gene was induced, suggesting a functional assignment of these homologues. RNA blot hybridization indicated a typical stress response of dnaK2 to heat and high-light stress. Primer extension mapping showed that dnaK2 was transcribed from similar sites under various stress conditions. Although no known sequence motif was detected in the upstream region, a 20-bp sequence element was highly conserved in dnaK2; it was essential not only for the stress induction but also for the basal expression of dnaK2. The ubiquitous upstream localization of this element in each heat shock gene suggests its important role in the cyanobacterial stress response.
摘要
我们分析了聚球藻属嗜热栖热菌PCC 7942中三个dnaK同源物（dnaK1、dnaK2和dnaK3）的应激反应。一项报告基因检测表明，在应激条件下，只有dnaK2基因的表达被诱导，这表明了这些同源物的功能分配。RNA印迹杂交表明dnaK2对热和高光应激有典型的应激反应。引物延伸图谱显示，dnaK2在各种应激条件下从相似位点转录。虽然在上游区域未检测到已知的序列基序，但一个20碱基对的序列元件在dnaK2中高度保守；它不仅对应激诱导至关重要，而且对dnaK2的基础表达也至关重要。该元件在每个热休克基因中普遍存在的上游定位表明其在蓝藻应激反应中的重要作用。
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