Kim H S, Park C B, Kim M S, Kim S C
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea.
Biochem Biophys Res Commun. 1996 Dec 13;229(2):381-7. doi: 10.1006/bbrc.1996.1814.
A cDNA containing coding information for buforin I, the toad stomach antimicrobial peptide, was identified by PCR. The cloned cDNA encoded a protein of 129 amino acids whose 39-amino-acid N-terminus was identical to buforin I. Nucleotide sequence analysis of the cloned cDNA revealed that it had over 90% amino acid homology with histone H2A, the replication-dependent protein. Both Northern and Southern blot analysis of the toad genome suggested that histone H2A and buforin I were encoded by the same gene. A specific protease responsible for the generation of buforin I from histone H2A was found to be present in the crude extracts of the toad stomach. These results suggest that there exists a specific regulation mechanism which converts the toad histone H2A to the antimicrobial peptide buforin I.
通过聚合酶链反应（PCR）鉴定出一个包含蟾蜍胃抗菌肽蟾蜍灵I编码信息的互补DNA（cDNA）。克隆的cDNA编码一种由129个氨基酸组成的蛋白质，其39个氨基酸的N端与蟾蜍灵I相同。对克隆的cDNA进行核苷酸序列分析表明，它与复制依赖性蛋白组蛋白H2A具有超过90%的氨基酸同源性。对蟾蜍基因组的Northern和Southern印迹分析均表明，组蛋白H2A和蟾蜍灵I由同一基因编码。在蟾蜍胃的粗提物中发现了一种负责从组蛋白H2A生成蟾蜍灵I的特异性蛋白酶。这些结果表明，存在一种将蟾蜍组蛋白H2A转化为抗菌肽蟾蜍灵I的特异性调控机制。
