噻唑烷二酮类胰岛素增敏剂BRL 49653可增加高脂喂养大鼠脂肪组织中PPAR-γ和aP2的表达。
The thiazolidinedione insulin sensitiser, BRL 49653, increases the expression of PPAR-gamma and aP2 in adipose tissue of high-fat-fed rats.
作者信息
Pearson S L, Cawthorne M A, Clapham J C, Dunmore S J, Holmes S D, Moore G B, Smith S A, Tadayyon M
机构信息
Clore Laboratory, University of Buckingham, Buckingham, MK18 1EG, United Kingdom.
出版信息
Biochem Biophys Res Commun. 1996 Dec 24;229(3):752-7. doi: 10.1006/bbrc.1996.1876.
The effects of the thiazolidinedione insulin sensitiser BRL 49653 on plasma leptin concentrations and on epididymal fat OB, PPAR-gamma and aP2 mRNA expression were examined in high-fat-fed and high-carbohydrate-fed adult Wistar rats. Diets were given for 4 weeks, with BRL 49653 (10 micromol/kg/day) administered by oral gavage for the last 4 days. Treatment with BRL 49653 reduced plasma leptin concentrations in high-fat-fed rats from 2.34 +/- 0.19 (n=9) to 1.42 +/- 0.09 (n=9) ng/ml (p<0.001). Plasma leptin was unaffected by BRL 49653 in the high-carbohydrate-fed rats. There was no difference in OB mRNA expression between high-fat-fed and high-carbohydrate-fed rats, with or without treatment. PPAR-gamma and aP2 mRNA expression were significantly increased in the high-fat-fed rats treated with BRL 49653 (p < 0.01 and p < 0.001 respectively), but not in carbohydrate-fed rats.
在高脂喂养和高碳水化合物喂养的成年Wistar大鼠中，研究了噻唑烷二酮胰岛素增敏剂BRL 49653对血浆瘦素浓度以及附睾脂肪中OB、PPAR-γ和aP2 mRNA表达的影响。给予大鼠4周饮食，在最后4天通过灌胃给予BRL 49653（10微摩尔/千克/天）。用BRL 49653治疗使高脂喂养大鼠的血浆瘦素浓度从2.34±0.19（n = 9）降至1.42±0.09（n = 9）纳克/毫升（p<0.001）。在高碳水化合物喂养的大鼠中，BRL 49653对血浆瘦素没有影响。无论是否接受治疗，高脂喂养和高碳水化合物喂养大鼠的OB mRNA表达均无差异。在用BRL 49653治疗的高脂喂养大鼠中，PPAR-γ和aP2 mRNA表达显著增加（分别为p < 0.01和p < 0.001），但在高碳水化合物喂养的大鼠中没有增加。
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