Begum N A, Coker A, Shibuta K, Swanson R S, Chen L B, Mori M, Barnard G F
Department of Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts, 01655, USA.
Biochem Biophys Res Commun. 1996 Dec 24;229(3):864-8. doi: 10.1006/bbrc.1996.1893.
We recently isolated from differential displays and subsequently cloned a human alpha-intercrine (hIRH) whose mRNA is reduced in human hepatocellular carcinomas. We now report that on Northern blots its mRNA is absent from premalignant colonic adenomas and from all of 27 human malignant cell lines (including breast, cervix, colon, duodenal, gastric, leukemia, liver, lung, melanoma, and pancreatic lines). hIRH mRNA was present in most normal human and mouse tissues and fibroblast derived cell lines but absent from leukocytes and brain. Two mRNA signals, at approximately 2 Kb and approximately 3.5 Kb, had variation in signal strength or size between tissues and species.
我们最近从差异显示中分离出一种人类α-内分泌素（hIRH），随后对其进行了克隆，该基因的mRNA在人类肝细胞癌中表达降低。我们现在报告，在Northern杂交中，癌前结肠腺瘤以及27种人类恶性细胞系（包括乳腺癌、宫颈癌、结肠癌、十二指肠癌、胃癌、白血病、肝癌、肺癌、黑色素瘤和胰腺癌细胞系）中均未检测到其mRNA。hIRH mRNA在大多数正常人类和小鼠组织以及成纤维细胞衍生的细胞系中存在，但在白细胞和脑中不存在。在大约2kb和大约3.5kb处的两个mRNA信号，在不同组织和物种之间信号强度或大小存在差异。
