Lamberti C, Weller S K
Department of Microbiology, University of Connecticut Health Center, Farmington 06030-3205, USA.
Virology. 1996 Dec 15;226(2):403-7. doi: 10.1006/viro.1996.0668.
The HSV-1 genome is composed of two unique regions (UL and US) flanked by inverted repeats. During the course of DNA replication the two unique regions UL and US invert relative to one another. In this report we present evidence that cleavage is not necessary for genomic inversion to occur. We isolated and characterized a UL6::lacZ insertion mutant (hr74) that produces wild-type levels of replicating viral DNA but fails to cleave and package DNA. We demonstrate that this virus is still able to undergo genomic inversion. Furthermore we confirm that replicating DNA from cells infected with wild-type virus contains specific UL terminal but not US termini, whereas cells infected with the mutant hr74 do not contain either US or UL termini. This demonstrates that the specific UL ends found in replicating DNA are the result of the cleavage/packaging process.
单纯疱疹病毒1型（HSV - 1）基因组由两个独特区域（UL和US）组成，两侧为反向重复序列。在DNA复制过程中，两个独特区域UL和US会相对彼此发生倒置。在本报告中，我们提供证据表明，基因组倒置的发生并不需要切割。我们分离并鉴定了一个UL6::lacZ插入突变体（hr74），它能产生野生型水平的复制病毒DNA，但无法切割和包装DNA。我们证明这种病毒仍然能够进行基因组倒置。此外，我们证实，感染野生型病毒的细胞中复制的DNA含有特定的UL末端而非US末端，而感染突变体hr74的细胞既不含有US末端也不含有UL末端。这表明在复制DNA中发现的特定UL末端是切割/包装过程的结果。
