Nalwanga D, Rempel S, Roizman B, Baines J D
Department of Microbiology and Immunology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853, USA.
Virology. 1996 Dec 15;226(2):236-42. doi: 10.1006/viro.1996.0651.
The UL16 gene of herpes simplex virus maps within the intron of the UL 15 gene. This report shows the following: (i) A polyclonal antiserum directed against a bacterial fusion protein containing glutathione S-transferase fused to the C-terminus of the UL 16 gene reacted with an apparent M(r) 40,000 protein in HSV-1 infected cell lysates. (ii) The protein encoded by UL 16 was dependent on viral DNA synthesis for accumulation to detectable levels. (iii) In immunofluorescence studies, the polyclonal UL 16/GST-specific antiserum was shown to stain the nucleus of infected cells at 18 hr after infection in areas containing high concentrations of HSV capsid proteins. These nuclear compartments have been described previously as viral assemblons (Ward et al., J. Virol. 70, 4623-4631, 1996) and are distinct from compartments containing replicating DNA. Localization within assemblons argues for a role of UL 16 encoded protein in capsid assembly or maturation. (iv) At 22 hr after infection, UL 16-specific immunofluorescence was present in both the nucleus and the cytoplasm. (v) Consistent with the change in localization at late times after infection, the UL 16 protein was found to be a component of purified virions.
单纯疱疹病毒的UL16基因定位于UL15基因的内含子内。本报告显示如下：(i) 一种针对细菌融合蛋白的多克隆抗血清，该融合蛋白包含与UL16基因C末端融合的谷胱甘肽S-转移酶，与单纯疱疹病毒1型感染细胞裂解物中一种表观分子量为40,000的蛋白发生反应。(ii) UL16编码的蛋白积累至可检测水平依赖于病毒DNA合成。(iii) 在免疫荧光研究中，多克隆UL16/GST特异性抗血清在感染后18小时显示可对感染细胞的细胞核进行染色，染色区域含有高浓度的单纯疱疹病毒衣壳蛋白。这些核区室先前已被描述为病毒装配体（Ward等人，《病毒学杂志》70，4623 - 4631，1996），且与含有正在复制的DNA的区室不同。在装配体内的定位表明UL16编码的蛋白在衣壳装配或成熟过程中发挥作用。(iv) 感染后2小时，细胞核和细胞质中均存在UL16特异性免疫荧光。(v) 与感染后期定位的变化一致，发现UL16蛋白是纯化病毒粒子的一个组成部分。
