大肠杆菌rpoC397编码RNA聚合酶β'亚基中一个温度敏感的C端移码突变，该突变阻碍噬菌体P2的生长。
Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2.
作者信息
Christie G E, Cale S B, Isaksson L A, Jin D J, Xu M, Sauer B, Calendar R
机构信息
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298-0678, USA.
出版信息
J Bacteriol. 1996 Dec;178(23):6991-3. doi: 10.1128/jb.178.23.6991-6993.1996.
Abstract
Escherichia coli 397c is temperature sensitive for growth at 43.5 degrees C and unable to plate bacteriophage P2 at 33 degrees C. The mutation conferring these phenotypes was mapped to the rpoC gene. RNA synthesis is temperature sensitive in the mutant strain, and the beta' subunit of RNA polymerase isolated from this strain exhibits increased electrophoretic mobility. DNA sequence analysis revealed that the mutation is a deletion of 16 bp, resulting in a frameshift that leads to truncation of the beta' subunit at the carboxy terminus.
摘要
大肠杆菌397c在43.5摄氏度时对生长温度敏感，且在33摄氏度时无法对噬菌体P2进行铺板。赋予这些表型的突变被定位到rpoC基因。在突变菌株中RNA合成对温度敏感，并且从该菌株中分离出的RNA聚合酶的β'亚基表现出增加的电泳迁移率。DNA序列分析表明该突变是一个16 bp的缺失，导致移码，从而导致β'亚基在羧基末端被截短。
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