Van Damme E J, Barre A, Verhaert P, Rougé P, Peumans W J
Laboratorium voor Fytopathologie en Plantenbescherming, Katholieke Universiteit Leuven, Belgium.
FEBS Lett. 1996 Nov 18;397(2-3):352-6. doi: 10.1016/s0014-5793(96)01211-2.
cDNA clones encoding the mitogenic mannose/maltose-specific lectin from the rhizomes of hedge bindweed (Calystegia sepium) have been isolated and sequenced. Comparison of the deduced amino acid sequence and the molecular weight of the lectin subunit as determined by mass spectrometry indicated that the mature protein comprises the entire open reading frame of the cDNA, which implies that the primary translation product contains no signal peptide and is not proteolytically processed. Searches in the databases revealed sequence homology with the previously described lectins from the taxonomically unrelated Moraceae species Artocarpus integrifolia and Maclura pomifera.
已分离并测序了编码来自篱打碗花（Calystegia sepium）根茎的促有丝分裂甘露糖/麦芽糖特异性凝集素的cDNA克隆。通过质谱法测定的凝集素亚基的推导氨基酸序列和分子量的比较表明，成熟蛋白包含cDNA的整个开放阅读框，这意味着初级翻译产物不包含信号肽且未经过蛋白水解加工。在数据库中搜索发现与之前描述的来自分类学上无关的桑科物种面包树（Artocarpus integrifolia）和桑橙（Maclura pomifera）的凝集素具有序列同源性。
