硝普盐试验用于胱氨酸尿症诊断的评估。

Evaluation of the nitroprusside test for the diagnosis of cystinuria.

作者信息

Smith A

出版信息

Med J Aust. 1977 Jul 30;2(5):153-5. doi: 10.5694/j.1326-5377.1977.tb99112.x.

DOI:10.5694/j.1326-5377.1977.tb99112.x

Abstract

Because of the wide acceptability of the nitroprusside test for the diagnosis of cystinuria, an analysis of 525 consecutive urine tests performed at this laboratory was undertaken, the nitroprusside test result being correlated with the urinary cystine excretion as determined by paper chromatography, high-voltage electrophoresis and chemical tests. It was found that the nitroprusside test clearly separated urine samples into categories of cystine excretion within the normal, heterozygote and homozygote range (P less than 0-001). However, 7 (1-33%) urine specimens from homozygous cystinurics gave a negative nitroprusside result. If properly performed on a concentrated urine specimen, this test is a very accurate means of diagnosing cystinuria (expected error approximately 1%), but it is not infallible.

摘要

由于硝普盐试验在胱氨酸尿症诊断中具有广泛的可接受性，因此对本实验室连续进行的525次尿液检测进行了分析，将硝普盐试验结果与通过纸色谱法、高压电泳法和化学检测法测定的尿胱氨酸排泄量进行关联。结果发现，硝普盐试验能将尿样清晰地分为正常、杂合子和纯合子范围内的胱氨酸排泄类别（P小于0.001）。然而，来自纯合性胱氨酸尿症患者的7份（1.33%）尿标本的硝普盐试验结果为阴性。如果对浓缩尿标本正确进行该试验，它是诊断胱氨酸尿症的一种非常准确的方法（预期误差约为1%），但并非万无一失。