Whalen R G
Centre National de la Recherche Scientifique, Collège de France, Paris, France.
Intervirology. 1996;39(1-2):120-5. doi: 10.1159/000150483.
Occasionally, a major change in vaccine methodology comes along. Such would appear to be the case with the advent of DNA-mediated immunization, colloquially known as DNA vaccines. This represents a radical new way to deliver antigens; it involves the direct introduction of a plasmid DNA encoding an antigenic protein which is then expressed within cells of the organism. This leads to surprisingly strong immune responses, involving both the humoral and cellular arms of the immune system. DNA-mediated immunization to a single antigen can provide protection against infection by a pathogen. Here, a guide is provided comprising twelve steps to help design and carry out DNA-mediated immunization. This approach to immunization will greatly facilitate studies of immunophysiological responses to antigens of pathogenic organisms. An Internet site (URL: http:@www.genweb.com/Dnavax/dnav ax.html) has been created to help promote this potentially revolutionary approach to vaccination in the service of public health.
偶尔，疫苗方法会出现重大变革。DNA介导免疫（俗称DNA疫苗）的出现似乎就是这种情况。这代表了一种全新的抗原递送方式；它涉及直接引入编码抗原蛋白的质粒DNA，该蛋白随后在机体细胞内表达。这会引发令人惊讶的强烈免疫反应，涉及免疫系统的体液免疫和细胞免疫分支。针对单一抗原的DNA介导免疫可提供针对病原体感染的保护。本文提供了一个包含十二个步骤的指南，以帮助设计和实施DNA介导免疫。这种免疫方法将极大地促进对致病生物体抗原的免疫生理反应的研究。已创建一个网站（网址：http:@www.genweb.com/Dnavax/dnavax.html），以帮助推广这种潜在的革命性疫苗接种方法，服务于公共卫生。
