Hamre K, Waagbø R, Berge R K, Lie O
Institute of Nutrition, Directorate of Fisheries, Bergen, Norway.
Free Radic Biol Med. 1997;22(1-2):137-49. doi: 10.1016/s0891-5849(96)00281-x.
A two way regression study was performed to investigate the interactions between vitamins C and E, and the influence of dietary vitamin C on the development of vitamin E deficiency in first feeding Atlantic salmon. The fish were fed three levels of all-rac-alpha-tocopheryl acetate (0, 150, and 300 mg/kg), each with six levels of ascorbate monophosphate (0, 7.5, 15, 30, 45, and 60 mg/kg ascorbic acid equivalents). Vitamin C protected the fish against vitamin E deficiency in a dose dependent manner, as seen from the data on growth, mortality, hematology, and lipid oxidation in the liver, indicated by the concentration of malondialdehyde. Vitamin C did not influence the tissue levels of vitamin E, except in vitamin C deficiency, which induced a large drop in liver vitamin E concentration. The liver level of vitamin C increased in response to supplementation of both vitamins. The results indicate two different interaction mechanisms: a synergistic effect of simultaneous protection of the water and lipid phases against oxidation, and regeneration of vitamin E from the vitamin E radical by ascorbic acid.
进行了一项双向回归研究，以调查维生素C和E之间的相互作用，以及日粮维生素C对初次摄食的大西洋鲑维生素E缺乏症发展的影响。给鱼投喂三种水平的消旋-α-生育酚醋酸酯（0、150和300毫克/千克），每种水平分别搭配六种水平的抗坏血酸单磷酸酯（0、7.5、15、30、45和60毫克/千克抗坏血酸当量）。从生长、死亡率、血液学以及肝脏中脂质氧化（以丙二醛浓度表示）的数据可以看出，维生素C以剂量依赖的方式保护鱼免受维生素E缺乏的影响。维生素C除了在维生素C缺乏时会导致肝脏维生素E浓度大幅下降外，对维生素E的组织水平没有影响。肝脏中维生素C的水平会因两种维生素的补充而升高。结果表明存在两种不同的相互作用机制：同时保护水相和脂质相免受氧化的协同作用，以及抗坏血酸将维生素E自由基再生为维生素E的作用。
