Nedergaard L, Christensen L, Rasmussen B B, Jacobsen G K
Department of Pathology, University Hospital, Denmark.
Pathol Res Pract. 1996 Oct;192(10):983-8. doi: 10.1016/S0344-0338(96)80039-2.
A new monoclonal antibody against estrogen receptor (ID5, Dako) has shown promising results when applied to formalin fixed, paraffin embedded tissue. In order to determine whether this antibody can compete in specificity and sensitivity with the ER-ICA antibody (Abbott Laboratories), a comparative, prospective study of the two antibodies was carried out on paraffin embedded and fresh frozen tissue in three laboratories. Two hundred and fifteen breast carcinomas were examined. Formalin fixed, paraffin embedded tissue was available from 215 tumors, and fresh frozen tissue from 189 of the tumors. Of these, 124 tumors were also investigated by the enzyme-linked immunosorbent assay. The results from each of the three laboratories correspond with those obtained for the whole material. The percentage of tumors positive for estrogen receptors within the different methods, was as follows: 71% by ID5 on paraffin sections, 50% by ER-ICA on paraffin sections, 65% ER-ICA on frozen sections and 88% by the EIA. When comparing the different immunohistochemical results and the EIA in 2 x 2 tables, agreement was reached in 69% to 91% of the cases. The best agreement (91%) was found between results obtained with the ID5 antibody used on formalin fixed, paraffin embedded tissue and the ER-ICA kit used on fresh frozen tissue. The advantages of using the ID5 antibody are associated with its applicability to formalin fixed, paraffin embedded tissue: improved morphology, reproducibility, retrospective studying and low costs. Finally, it is reproducible not only within the same laboratory but also among different laboratories.
一种针对雌激素受体的新型单克隆抗体（ID5，达科公司）应用于福尔马林固定、石蜡包埋组织时已显示出有前景的结果。为了确定该抗体在特异性和敏感性方面是否能与ER-ICA抗体（雅培实验室）相媲美，在三个实验室对这两种抗体在石蜡包埋组织和新鲜冷冻组织上进行了一项对比性前瞻性研究。共检查了215例乳腺癌。从215个肿瘤中获取了福尔马林固定、石蜡包埋组织，从189个肿瘤中获取了新鲜冷冻组织。其中，124个肿瘤还通过酶联免疫吸附测定法进行了研究。三个实验室各自的结果与对整个样本获得的结果一致。不同方法检测雌激素受体阳性的肿瘤百分比如下：石蜡切片上ID5法为71%，石蜡切片上ER-ICA法为50%，冷冻切片上ER-ICA法为65%，酶联免疫吸附测定法为88%。在2×2表格中比较不同免疫组织化学结果和酶联免疫吸附测定法时，69%至91%的病例达成了一致。在用福尔马林固定、石蜡包埋组织上使用ID5抗体获得的结果与在新鲜冷冻组织上使用ER-ICA试剂盒获得的结果之间发现了最佳一致性（91%）。使用ID5抗体的优势与其适用于福尔马林固定、石蜡包埋组织有关：形态改善、可重复性、回顾性研究以及成本低。最后，它不仅在同一实验室内可重复，而且在不同实验室之间也可重复。
