Watanabe M, Asakura K, Saito H, Kataura A
Department of Otolaryngology School of Medicine, Sapporo Medical University.
Arerugi. 1996 Oct;45(10):1127-32.
For the purpose of making a murine model of nasal allergy, we performed the ovalbumin (OA)-sensitization on groups of BALB/c mice in four different ways. The 1st group was sensitized with OA + alum by introperitoneal myection on day 1, 5 and 21, and the 2nd group was added OA nasal drips on day 28, 34. The 3rd group was performed OA + alum nasal dripping on day 1, 5 and 21, and the 4th was a added OA nasal dripping in the same way with the 2nd group. The observed OA-induced nasal symptoms, mucosal eosinophillia and 8 days-PCA titer were remarkable in 2nd group, moreover it also showed higher histamine hypersensitivity than other groups. We concluded that mice sensitized by OA with alum are useful for research of nasal allergy, for the reason that they posses three features of nasal allergy, anti-intigen-induced nasal symptoms, eosinophils infiltration and antigen-specific PCA titer as index of IgE increasing.
为了制作小鼠鼻腔过敏模型，我们以四种不同方式对多组BALB/c小鼠进行卵清蛋白（OA）致敏。第一组在第1、5和21天通过腹腔注射用OA+明矾致敏，第二组在第28、34天添加OA滴鼻。第三组在第1、5和21天进行OA+明矾滴鼻，第四组以与第二组相同的方式添加OA滴鼻。观察到第二组中OA诱导的鼻腔症状、黏膜嗜酸性粒细胞增多和8天被动皮肤过敏反应（PCA）滴度显著，此外其组胺超敏反应也高于其他组。我们得出结论，用OA加明矾致敏的小鼠可用于鼻腔过敏研究，因为它们具有鼻腔过敏的三个特征，即抗抗原诱导的鼻腔症状、嗜酸性粒细胞浸润以及作为IgE增加指标的抗原特异性PCA滴度。
