Wassberg E, Stridsberg M, Christofferson R
Department of Human Anatomy, University of Uppsala, Sweden.
J Endocrinol. 1996 Nov;151(2):225-30. doi: 10.1677/joe.0.1510225.
A novel animal experimental model involving the human, poorly differentiated, and adrenergic neuroblastoma cell line SH-SY5Y xenotransplanted to subcutaneous tissue of 13 nude rats (WAG rnu/rnu) was used to investigate the usefulness of six proposed neuroblastoma markers. It was shown that the plasma concentrations of human chromogranin A (CgA) as measured by RIA were directly proportional to tumour volume (r = 0.83, P < 0.001). To rule out possible liberation of CgA by tumour cell lysis, the CgA degradation product pancreastatin was also measured in plasma by a specific RIA, but was not detectable. Plasma neurone-specific enolase (NSE) was elevated in tumour-bearing animals (P < 0.01), but did not correlate with tumour volume (r = 0.49, P > 0.05). Urine homovanillic acid (HVA), detected by HPLC, was elevated in tumour-bearing animals (P < 0.01), but did not correlate with tumour volume (r = -0.32, P > 0.05). Urine vanillyl mandelic acid was not detectable. Urine dopamine was found in low concentrations that did not correlate with tumour volume. In summary, although plasma NSE and urinary HVA were elevated in tumour-bearing animals only plasma CgA correlated with tumour burden. This makes CgA a promising biochemical marker for neuroblastomas.
采用一种新的动物实验模型，即将人低分化肾上腺能神经母细胞瘤细胞系SH-SY5Y异种移植到13只裸鼠（WAG rnu/rnu）的皮下组织中，以研究六种提出的神经母细胞瘤标志物的实用性。结果表明，放射免疫分析法测定的人嗜铬粒蛋白A（CgA）血浆浓度与肿瘤体积成正比（r = 0.83，P < 0.001）。为排除肿瘤细胞裂解可能释放CgA的情况，还通过特异性放射免疫分析法在血浆中测定了CgA降解产物胰抑制素，但未检测到。荷瘤动物血浆神经元特异性烯醇化酶（NSE）升高（P < 0.01），但与肿瘤体积无相关性（r = 0.49，P > 0.05）。高效液相色谱法检测发现，荷瘤动物尿高香草酸（HVA）升高（P < 0.01），但与肿瘤体积无相关性（r = -0.32，P > 0.05）。尿香草扁桃酸未检测到。发现尿多巴胺浓度较低，与肿瘤体积无相关性。总之，虽然荷瘤动物血浆NSE和尿HVA升高，但只有血浆CgA与肿瘤负荷相关。这使得CgA成为一种有前景的神经母细胞瘤生化标志物。
