Naderali E K, Poyser N L
Department of Pharmacology, University of Edinburgh Medical School, UK.
J Reprod Fertil. 1996 Sep;108(1):75-80. doi: 10.1530/jrf.0.1080075.
Using guinea-pig uterine tissues, indomethacin (a non-selective inhibitor of prostaglandin H synthase) inhibited prostaglandin (PG) synthesis by homogenates of the endometrium, by cultured endometrium and myometrium, and by cultured epithelial glandular cells and stromal cells derived from the endometrium. NS-398, a selective inhibitor of prostaglandin H synthase-2 (PGHS-2), also inhibited PG synthesis by endometrial homogenates, by cultured endometrium and myometrium, and by cultured epithelial glandular cells and stromal cells. Indomethacin and NS-398 inhibited PG production to similar extents, except for 6-keto-PGF1 alpha production by the myometrium where indomethacin was more effective. In particular, indomethacin and NS-398 produced over 90% inhibition of PGF2 alpha output from the epithelial glandular cells, the main source of PGF2 alpha in the endometrium. These functional studies indicate that prostaglandin H synthase-2 is the predominant PG-forming enzyme in the guinea-pig uterus.
使用豚鼠子宫组织，吲哚美辛（一种前列腺素H合成酶的非选择性抑制剂）可抑制子宫内膜匀浆、培养的子宫内膜和子宫肌层以及源自子宫内膜的培养上皮腺细胞和基质细胞中前列腺素（PG）的合成。NS - 398，一种前列腺素H合成酶 - 2（PGHS - 2）的选择性抑制剂，也可抑制子宫内膜匀浆、培养的子宫内膜和子宫肌层以及培养的上皮腺细胞和基质细胞中PG的合成。吲哚美辛和NS - 398对PG产生的抑制程度相似，但子宫肌层中6 - 酮 - PGF1α的产生除外，在这方面吲哚美辛更有效。特别是，吲哚美辛和NS - 398对上皮腺细胞中PGF2α的输出产生了超过90%的抑制，上皮腺细胞是子宫内膜中PGF2α的主要来源。这些功能研究表明，前列腺素H合成酶 - 2是豚鼠子宫中主要的PG形成酶。
