Bodnar J W, Bradley M K
Chemistry Department, U.S. Naval Academy, Annapolis, MD 21402, USA. Bodnar(a Brass.NADN.Navy.Mil
J Theor Biol. 1996 Nov 7;183(1):1-7. doi: 10.1006/jtbi.1996.0195.
Cellular and molecular biological approaches that study eukaryotic gene regulation have led to separate models which describe structure and mechanism with differing precision. Using principles of combinatorial and cooperative interactions inherent in both models, we have extended concepts derived from a "genetic switch" for prokaryotes to a chromatin switch for eukaryotes composed of DNA, transactivators, nucleosomes and the nuclear matrix. We present a consensus model for gene regulation that uses a simple Monte Carlo method for simulating condensation and extension of chromatin. Such a chromatin switch can be modulated by known biochemical and molecular modifications, and the transactivator binding sites or enhancers within DNA domains can be organized into a hierarchy to control cell cycling and differentiation.
研究真核基因调控的细胞和分子生物学方法产生了不同的模型，这些模型对结构和机制的描述精度各异。利用这两种模型中固有的组合和协同相互作用原理，我们将源自原核生物“遗传开关”的概念扩展到了由DNA、反式激活因子、核小体和核基质组成的真核生物染色质开关。我们提出了一个基因调控的共识模型，该模型使用一种简单的蒙特卡罗方法来模拟染色质的凝聚和伸展。这样的染色质开关可以通过已知的生化和分子修饰进行调节，并且DNA结构域内的反式激活因子结合位点或增强子可以组织成一个层次结构来控制细胞周期和分化。
