Chen C, Madeleine M M, Lubinski C, Weiss N S, Tickman E W, Daling J R
Public Health Sciences Core Laboratory in Program in Cancer Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Cancer Epidemiol Biomarkers Prev. 1996 Dec;5(12):985-91.
Some studies have reported an association of the GSTM1-null genotype with the risk of smoking-related cancers, such as lung, bladder, and colon cancer. Because the risk of anal cancer is strongly associated with a history of cigarette smoking, we examined whether the GSTM1-null genotype is a susceptibility marker for anal cancer. We obtained peripheral blood specimens from residents of western Washington who were diagnosed with squamous or transitional cell tumor of the anus between April 1991 and June 1994. Eligible for inclusion were persons 18-74 years of age, with either invasive or in situ lesions. Specimens were also obtained from controls identified via random-digit dialing of western Washington households. We determined GSTM1 genotypes of 71 cases and 360 controls by PCR using primer pair 5'-AACTCCCTGAAAAGCTAAAGC-3' and 5'-GTTGGGCTCAAATATACGGTGG-3'. The frequency of the GSTM1-null genotype in controls was approximately 57%; this differed little in relation to age, sex and smoking status. The incidence of anal cancer appeared to be reduced in persons with the GSTM1-null genotype; only 39.4% of cases had this genotype (age-adjusted odds ratio = 0.5, 95% confidence interval = 0.3-0.9). This inverse association was restricted to persons who had ever smoked cigarettes and was present in both women and men (and in the latter, in both those who had and did not have a male sexual partner). Our data strongly suggest that persons with the GSTM1-null genotype are not at increased risk of anal cancer, and may well be at a decreased risk.
一些研究报告称，谷胱甘肽S-转移酶M1（GSTM1）基因缺失型与吸烟相关癌症（如肺癌、膀胱癌和结肠癌）的风险存在关联。由于肛管癌的风险与吸烟史密切相关，我们研究了GSTM1基因缺失型是否是肛管癌的易感性标志物。我们从1991年4月至1994年6月期间被诊断患有肛管鳞状或移行细胞肿瘤的华盛顿州西部居民中获取外周血标本。纳入标准为年龄在18至74岁之间、患有浸润性或原位病变的患者。还通过随机拨打华盛顿州西部家庭的电话号码确定了对照样本。我们使用引物对5'-AACTCCCTGAAAAGCTAAAGC-3'和5'-GTTGGGCTCAAATATACGGTGG-3'通过聚合酶链反应（PCR）测定了71例患者和360例对照的GSTM1基因型。对照中GSTM1基因缺失型的频率约为57%；在年龄、性别和吸烟状况方面差异不大。GSTM1基因缺失型患者的肛管癌发病率似乎有所降低；只有39.4%的病例具有这种基因型（年龄调整后的优势比=0.5，95%置信区间=0.3-0.9）。这种负相关仅限于曾经吸烟的人群，在女性和男性中均存在（在男性中，无论是否有男性性伴侣）。我们的数据强烈表明，GSTM1基因缺失型个体患肛管癌的风险并未增加，反而很可能降低。
