Tonokatsu Y, Fukuda Y, Tsujiai T, Tamura K, Shimoyama T
Fourth Department of Internal Medicine, Mukogawa, Japan.
J Gastroenterol. 1996 Nov;31 Suppl 9:75-6.
Eradication therapy for Helicobacter pylori is now common, since eradication of this organism reduces the recurrence rate of peptic ulcer. We evaluated the eradication of H. pylori 4 weeks after the cessation of eradication treatment, and encountered recolonization in patients in whom eradication had been confirmed. Using a urease probe, we analyzed the Ure B restriction fragment length polymorphism (RFLP) of the DNA of the recolonized H. pylori by Southern blotting and compared it with that of the H. pylori strain cultured before eradication therapy. RFLP patterns before and after the treatment were identical, indicating that the recolonization of H. pylori was an exacerbation of the primary infection, rather than a reinfection.
由于根除幽门螺杆菌可降低消化性溃疡的复发率，因此幽门螺杆菌根除疗法现已普遍应用。我们在根除治疗停止4周后评估幽门螺杆菌的根除情况，发现在已确认根除的患者中出现了重新定植。我们使用尿素酶探针，通过Southern印迹法分析重新定植的幽门螺杆菌DNA的尿素酶B限制片段长度多态性（RFLP），并将其与根除治疗前培养的幽门螺杆菌菌株的RFLP进行比较。治疗前后的RFLP模式相同，表明幽门螺杆菌的重新定植是原发性感染的加重，而非再次感染。
