移植端脑中含波形蛋白的放射状胶质细胞被含胶质纤维酸性蛋白的星形胶质细胞快速替代。
A rapid replacement of vimentin-containing radial glia by glial fibrillary acidic protein-containing astrocytes in transplanted telencephalon.
作者信息
Tuba A, Kállai L, Kálmán M
机构信息
Department of Anatomy, Histology, and Embryology, Semmelweis University of Medicine, Budapest, Hungary.
出版信息
J Neural Transplant Plast. 1997 Jan-Mar;6(1):21-9. doi: 10.1155/NP.1997.21.
The present study follows the early events in the development of astroglia in rat embryonic (E15) tissue grafted into the cortex of adult rats. Astroglial elements (radial glia and astrocytes) were studied by glial fibrillary acidic protein (GFAP) and vimentin immunohistochemistry on post-transplantation (PT) days 7, 11, 14, 17, and 21. At PT7, GFAP-immunopositive elements were only scarce fibers in the transplants. At PT11, a dense network of long, GFAP-immunopositive fibers enmeshed the entire transplant, and astrocytes were already recognized. The fibers also showed vimentin immunoreactivity. By PT14, astrocytes became the predominant GFAP-labeled elements, although a few long fibers persisted. When compared with in situ development, the grafts showed earlier GFAP-immunoreactivity and earlier appearance of astrocytes, as well as a more rapid transition from the immature to the mature form of the glial system.
本研究追踪了移植到成年大鼠皮质中的大鼠胚胎（E15）组织中星形胶质细胞发育的早期事件。在移植后（PT）第7、11、14、17和21天，通过胶质纤维酸性蛋白（GFAP）和波形蛋白免疫组织化学对星形胶质细胞成分（放射状胶质细胞和星形胶质细胞）进行了研究。在PT7时，GFAP免疫阳性成分在移植组织中仅为稀少的纤维。在PT11时，密集的长GFAP免疫阳性纤维网络环绕着整个移植组织，并且已经识别出星形胶质细胞。这些纤维也显示波形蛋白免疫反应性。到PT14时，星形胶质细胞成为GFAP标记的主要成分，尽管仍有一些长纤维存在。与原位发育相比，移植组织显示出更早的GFAP免疫反应性和更早出现的星形胶质细胞，以及从胶质系统的未成熟形式到成熟形式的更快转变。
Zotero 插件