Shostak A, Pivnik E, Gotloib L
Department of Nephrology and Hypertension, Central Emek Hospital, Afula, Israel.
J Am Soc Nephrol. 1996 Nov;7(11):2371-8. doi: 10.1681/ASN.V7112371.
This study was designed to examine whether rat peritoneal mesothelial cells in culture could generate hydrogen peroxide in different experimental conditions. Mesothelial cells, incubated in M-199, spontaneously released hydrogen peroxide. This process was significantly increased by addition of phorbol myristate acetate, as well as of superoxide dismutase to the medium, whereas it was substantially inhibited by catalase. Exposure of mesothelial cells to modified M-199 medium with 1.5% glucose concentration-lactated peritoneal dialysis solution did not seem to interfere either with the spontaneous release of hydrogen peroxide, or with that induced by phorbol myristate acetate. Furthermore, exposure of mesothelial cells to the glucose (4.25%) peritoneal dialysis solution in Medium M-199, was coincident with increased hydrogen peroxide generation, which was significantly higher than the spontaneous release, and not far from that observed with phorbol myristate acetate and superoxide dismutase. So far, it can be inferred from this evidence that peritoneal mesothelial cells in culture are not only endowed with the capability of producing hydrogen peroxide, but they can also be activated to do so in a way comparable to that observed in neutrophils and macrophages. This attribute is one more indication that mesothelial cells play a relevant role in the peritoneal mechanism of defense against infection. On the other hand, continuous exposure of mesothelial cells to glucose-enriched fluids, as occurs in clinical continuous ambulatory peritoneal dialysis, may well also be at the origin of a process of continuous injury, resulting from an increased hydrogen peroxide generation.
本研究旨在检测培养的大鼠腹膜间皮细胞在不同实验条件下是否能产生过氧化氢。在M - 199培养基中孵育的间皮细胞会自发释放过氧化氢。加入佛波酯肉豆蔻酸酯以及超氧化物歧化酶到培养基中，这一过程会显著增强，而过氧化氢酶则会对其产生显著抑制。将间皮细胞暴露于含1.5%葡萄糖浓度的乳酸盐腹膜透析液的改良M - 199培养基中，似乎既不影响过氧化氢的自发释放，也不影响佛波酯肉豆蔻酸酯诱导的释放。此外，将间皮细胞暴露于M - 199培养基中的葡萄糖（4.25%）腹膜透析液中，会伴随过氧化氢生成增加，这显著高于自发释放量，且与佛波酯肉豆蔻酸酯和超氧化物歧化酶诱导的生成量相近。目前，从这些证据可以推断，培养的腹膜间皮细胞不仅具备产生过氧化氢的能力，而且还能以类似于中性粒细胞和巨噬细胞的方式被激活来产生过氧化氢。这一特性进一步表明间皮细胞在腹膜抗感染防御机制中发挥着重要作用。另一方面，正如临床持续性非卧床腹膜透析中发生的那样，间皮细胞持续暴露于富含葡萄糖的液体中，很可能也是由于过氧化氢生成增加导致持续损伤过程的根源。
