Miyazaki M, Abe K, Koji T, Furusu A, Ozono Y, Harada T, Nakane P K, Yagame M, Endoh M, Nomoto Y, Sakai H
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
J Am Soc Nephrol. 1996 Nov;7(11):2428-33. doi: 10.1681/ASN.V7112428.
Complements 3 and 4 are known to be synthesized in diseased renal tissue and the mRNA of these complements has been demonstrated, using polymerase chain reaction, in renal biopsies from nephritic patients. However, the types of cells producing the complements in intact renal tissue have not been defined. To identify the renal cellular components involved in complement synthesis, we analyzed the expression of C3 mRNA in renal tissues from patients with immune-complex glomerulonephritis by a high-resolution in situ hybridization using digoxigenin-labeled oligonucleotide. Renal tissues from 15 patients with immunoglobulin (Ig) A nephropathy (IgAN), five with lupus nephritis (LN), and five with minimal change nephrotic syndrome (MCNS) were examined. Uninvolved portions of surgically removed kidney with tumors served as normal controls. C3 mRNA was detected in mesangial cells, glomerular epithelial cells, and Bowman's capsule in IgAN and LN. In the interstitium, some tubules and some infiltrating mononuclear cells were positively stained for C3 mRNA. C3 mRNA was not detected in MCNS and control tissues. Our results confirm that the glomerular resident cells can synthesize C3 in immune-mediated glomerulonephritis and suggest that locally synthesized complement may be involved in tissue injury in glomerulonephritis.
已知补体3和补体4在病变的肾组织中合成，并且利用聚合酶链反应已在肾炎患者的肾活检组织中证实了这些补体的信使核糖核酸（mRNA）。然而，完整肾组织中产生补体的细胞类型尚未明确。为了鉴定参与补体合成的肾细胞成分，我们通过使用地高辛标记的寡核苷酸进行高分辨率原位杂交，分析了免疫复合物性肾小球肾炎患者肾组织中C3 mRNA的表达。检测了15例免疫球蛋白A肾病（IgAN）患者、5例狼疮性肾炎（LN）患者和5例微小病变肾病综合征（MCNS）患者的肾组织。手术切除的伴有肿瘤的肾脏未受累部分用作正常对照。在IgAN和LN患者中，系膜细胞、肾小球上皮细胞和鲍曼囊检测到C3 mRNA。在间质中，一些肾小管和一些浸润的单核细胞C3 mRNA呈阳性染色。在MCNS和对照组织中未检测到C3 mRNA。我们的结果证实，在免疫介导的肾小球肾炎中，肾小球固有细胞能够合成C3，并提示局部合成的补体可能参与了肾小球肾炎的组织损伤。
