利用密度梯度离心法分离犬传染性肉瘤（CTS）中的活细胞。

Isolation of viable cells in canine transmissible sarcoma (CTS) using density gradient centrifugation.

作者信息

Okamoto Y, Minami S, Matsuhashi A, Fujinaga T

机构信息

Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, Japan.

出版信息

J Vet Med Sci. 1996 Nov;58(11):1149-51. doi: 10.1292/jvms.58.11_1149.

DOI:10.1292/jvms.58.11_1149

PMID:8959669

Abstract

Density gradient centrifugation using Ficoll-Conray solution was attempted to isolate viable canine transmissible sarcoma (CTS) cells. The viability of isolated tumor cells increased from about 50% to > 90%, and the yield of CTS cells was > 50% with over 99% purity, in when an isolation solution density of 1.05 was used.

摘要

尝试使用聚蔗糖-泛影葡胺溶液进行密度梯度离心来分离存活的犬传染性肉瘤（CTS）细胞。当使用密度为1.05的分离溶液时，分离出的肿瘤细胞活力从约50%提高到>90%，CTS细胞产量>50%，纯度超过99%。