Pascher A, Poehlein C, Storck M, Abendroth D, Mueller-Hoecker J, Koenig W, Young V K, White D J, Hammer C
Institute for Surgical Research, Klinikum Grosshadern, LMU Munich, Germany.
Transpl Int. 1996;9 Suppl 1:S385-7. doi: 10.1007/978-3-662-00818-8_93.
Ex vivo perfusions of human decay accelerating factor-expressing transgenic (n = 3), and nontransgenic (n = 6) porcine livers with human blood revealed a higher degree of organ damage in non-transgenic pig livers. Transgenic livers were protected from immunohistologically detectable complement deposition, despite corresponding IgM and IgG deposits in both groups. Complement activation and consumption of C3 and C4 turned out to be lower in transgenic pig livers. In contrast to livers of normal landrace pigs, livers from genetically manipulated pigs showed no morphological alterations after perfusion.
用人血对表达人衰变加速因子的转基因猪肝脏（n = 3）和非转基因猪肝脏（n = 6）进行离体灌注，结果显示非转基因猪肝脏的器官损伤程度更高。尽管两组均有相应的IgM和IgG沉积，但转基因肝脏免受免疫组织学可检测到的补体沉积影响。结果表明，转基因猪肝脏中的补体激活以及C3和C4的消耗较低。与正常长白猪的肝脏不同，基因操作猪的肝脏在灌注后未显示形态学改变。
