Akerboom T P, Ji Y, Wagner G, Sies H
Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, F.R.G.
Biochem Pharmacol. 1997 Jan 10;53(1):117-20. doi: 10.1016/s0006-2952(96)00602-8.
Glutathione transferase (GST)-catalysed S-nitrosoglutathione (GSNO) formation from alkyl nitrites was determined with the homodimers 1-1, 2-2, 3-3, and 4-4 isolated from rat liver. The 4-4 isoform showed a high specificity for the alkyl nitrites. Total GST activities were studied in homogenates from different organs. The liver showed highest GST activity both with amyl nitrite and with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, the activity ratio of amyl nitrite over CDNB being 3.8. In lung and heart, these ratios were 6.2 and 5.7, respectively, indicating a selectivity of these organs for alkyl nitrite metabolism and GSNO formation.
