小鼠髓过氧化物酶基因包含一个双组分远端增强子，其中包括一个受PEBP2/CBF调控的新区域。

The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF.

作者信息

Friedman A D, Britos-Bray M, Suzow J

机构信息

Johns Hopkins Oncology Center, Division of Pediatric Oncology, Baltimore, MD 21287, USA.

出版信息

Leuk Res. 1996 Oct;20(10):809-15. doi: 10.1016/s0145-2126(96)00035-5.

DOI:10.1016/s0145-2126(96)00035-5

PMID:8960105

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP2/CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA.

摘要

髓过氧化物酶（MPO）基因在未成熟髓样细胞中特异性表达。从小鼠MPO基因获得的DNA（27 kb）被亚克隆为九个片段，并检测每个片段增强32D cl3髓样细胞中转录的能力。除了已知的近端增强子外，只有一个片段具有活性。对该区域进行缺失分析确定了两个功能区。一个位于近端增强子上游3.4 kb处，最近在另一个细胞系统中也被鉴定出来。第二个新区域是位于上游1.1 kb处的一个133 bp片段。该区域与近端增强子一样，能结合PEBP2/CBF并受其调控。远端增强子中的PEBP2/CBF结合位点AACCAAT与PEBP2/CBF共有序列PuACCPuCA不同。