Suchocka Z, Kobylińska K, Pachecka J
Department of Biochemistry and Clinical Chemistry, Faculty of Pharmacy, School of Medicine, Warsaw, Poland.
Acta Pol Pharm. 1995 May-Jun;52(3):207-11.
Cytosolic liver glutathione S-transferase (GST) activity was decreased for CDNB and DCNB as substrates in long term alloxan induced diabetes. Similar to cytosolic, microsomal glutathione S-transferase activity was also decreased for CDNB. In contrast, both microsomal and cytosolic GST activities for ETA as well as cytosolic and microsomal glutathione (GSH) contents were unaffected. The activity of Se-dependent glutathione peroxidase activity, but not nonSe-dependent peroxidase activity was increased in diabetic rats. The results suggest that diabetic state has a different effect on each isoenzyme of hepatic glutathione S-transferase activity. After insulin treatment of diabetic animals the activities of both cytosolic and microsomal GST was not restored and the activity of non Se-GSHPx was significantly lower than the control value.
在长期用四氧嘧啶诱导的糖尿病模型中，以1-氯-2,4-二硝基苯（CDNB）和1,2-二氯-4-硝基苯（DCNB）为底物时，胞质肝谷胱甘肽S-转移酶（GST）活性降低。与胞质情况相似，以CDNB为底物时，微粒体谷胱甘肽S-转移酶活性也降低。相比之下，以1,2-环氧-3-（对硝基苯氧基）丙烷（ETA）为底物时，微粒体和胞质GST活性以及胞质和微粒体谷胱甘肽（GSH）含量均未受影响。糖尿病大鼠中硒依赖性谷胱甘肽过氧化物酶活性增加，但非硒依赖性过氧化物酶活性未增加。结果表明，糖尿病状态对肝谷胱甘肽S-转移酶活性的每种同工酶都有不同影响。对糖尿病动物进行胰岛素治疗后，胞质和微粒体GST的活性均未恢复，且非硒依赖性谷胱甘肽过氧化物酶（non Se-GSHPx）的活性显著低于对照值。
