Chromatographic analysis (TLC) of uracil arabinoside, cytosine arabinoside, uracil and cytosine in human plasma.

Cytosine arabinoside, uracil arabinoside, cytosine and uracil after solid-phase extraction from plasma with minicolumns Adsorbex were separated by TLC on silica gel 60 GF254 by ascending and horizontal technique using suitable mobile phases. The substances were identified by UV irradiation at 254 nm and by spraying of 1% KMnO4 solution (up to the amount 500 ng analysed substances).