Wierzchowski J, Wroczyński P, Interewicz E
Department of Physical Chemistry, Faculty of Pharmacy, Warsaw School of Medicine, Poland.
Acta Pol Pharm. 1996 May-Jun;53(3):203-8.
Continuous fluorimetric assay for aldehyde dehydrogenase activity in rat tissue, based on oxidation of the fluorogenic 6-methoxy-2-naphthaldehyde, is described. The new assay is ca. 2 orders of magnitude more sensitive than the standard procedures and it is not subjected to interferences by other dehydrogenases, although occasionally the aldehyde dehydrogenase activity may be obscured by aldehyde oxidase. The new fluorimetric assay is highly selective for the cytosolic forms of the both rat and human aldehyde dehydrogenase, which are primarily responsible for cyclophosphamide inactivation in vivo. These forms can now be detected in crude homogenates of several rat organs without necessity of subcelluar fractionation.
本文描述了一种基于荧光底物6-甲氧基-2-萘甲醛氧化反应的大鼠组织中醛脱氢酶活性的连续荧光测定法。新方法的灵敏度比标准方法高约2个数量级，且不受其他脱氢酶的干扰，不过醛脱氢酶活性偶尔可能会被醛氧化酶掩盖。这种新的荧光测定法对大鼠和人醛脱氢酶的胞质形式具有高度选择性，这些形式在体内主要负责环磷酰胺的失活。现在无需进行亚细胞分级分离，就能在几种大鼠器官的粗匀浆中检测到这些形式。
