Krantis A, Glasgow I, McKay A E, Mattar K, Johnson F
Department of Physiology, University of Ottawa, ON, Canada.
Can J Physiol Pharmacol. 1996 Aug;74(8):894-903.
The normal gut exhibits both contractions and relaxation from a resting tonus. Pathological and pharmacological information may be gleaned from separate measurement of these activities. Methods for recording and analyzing gut contractions have been presented before. We present an extension to the methods, utilising simple foil strain gauges tested here in male Sprague-Dawley rats. Coupled with a computer-based data acquisition and analysis system, we could assess the relaxation and contraction waves recorded simultaneously and from different gut regions in vivo. In contrast with the antrum, where spontaneous motor activity consisted of low frequency relaxations and random contractions, spontaneous duodenal motility was patterned into periodic groups of intense activity interposed by periods of low amplitude, low frequency contractions and relaxations. This grouped activity was propagatory, reminiscent of migrating myoelectrical complexes. When challenged with the ulcerogen cysteamine-HCl (56 mg/100 g s.c.), only duodenal motor activity was affected. Moreover, this treatment had differential effects in the duodenum. Patterned motility was no longer distinguishable, and contraction frequency and amplitude were increased while relaxation amplitude was decreased. This method affords a particularly sensitive and more precise assessment of both contractile and relaxant motor activity in vivo, before and after drug treatment.
正常肠道在静息张力基础上会出现收缩和舒张。这些活动的单独测量可以获取病理和药理学信息。此前已有记录和分析肠道收缩的方法。我们在此介绍这些方法的扩展，利用在雄性Sprague-Dawley大鼠中测试的简单箔式应变片。结合基于计算机的数据采集和分析系统，我们能够评估在体同时从不同肠道区域记录到的舒张波和收缩波。与胃窦不同，胃窦的自发运动活动由低频舒张和随机收缩组成，十二指肠的自发运动则表现为周期性的强烈活动组，其间夹杂着低幅度、低频的收缩和舒张。这种分组活动具有传播性，类似于移行性肌电复合波。当用致溃疡剂半胱胺盐酸盐（56毫克/100克皮下注射）刺激时，仅十二指肠运动活动受到影响。此外，这种处理在十二指肠有不同的作用。有规律的运动不再可辨，收缩频率和幅度增加，而舒张幅度减小。这种方法能够在药物治疗前后对在体的收缩性和舒张性运动活动进行特别灵敏且更精确的评估。
