Ekström J, Asztély A, Tobin G
Department of Pharmacology, Göteburg University, Sweden.
Exp Physiol. 1996 Nov;81(6):935-42. doi: 10.1113/expphysiol.1996.sp003994.
In pentobarbitione-anaesthetized rats the parasympathetic auriculotemporal nerve of the parotid gland was continuously stimulated at supramaximal voltage and at maximal frequency (40 Hz) for salivary secretion. The animals were pretreated with phentolamine and propranolol (2 mg kg-1 i.p. of each) and, in some groups, additionally with atropine (2 mg kg-1 i.p.). Morphometric assessment at the light microscopic level (x 100) showed that the numerical density of parotid acinar secretory granules (per 100 microns2 acinar epithelial cytoplasm) was reduced by 30 and 39% after 40 and 80 min, respectively, of stimulation in non-atropinized animals and by 30 and 27% in atropinized animals. The numerical density of acinar granules was not influenced by pretreatment with the protein synthesis inhibitor cycloheximide. The results suggest that most of the parasympathetic nerve-induced degranulation in the absence of muscarinic receptor blockade can be attributed to the action of non-adrenergic, non-cholinergic mechanisms.
在戊巴比妥麻醉的大鼠中，以超最大电压和最大频率（40赫兹）持续刺激腮腺的副交感耳颞神经以促进唾液分泌。动物预先用酚妥拉明和普萘洛尔（每种2毫克/千克腹腔注射）进行处理，并且在一些组中，另外还用阿托品（2毫克/千克腹腔注射）进行处理。在光学显微镜水平（×100）进行的形态计量学评估显示，在未用阿托品处理的动物中，刺激40分钟和80分钟后，腮腺腺泡分泌颗粒的数量密度（每100平方微米腺泡上皮细胞质）分别降低了30%和39%，在用阿托品处理的动物中分别降低了30%和27%。腺泡颗粒的数量密度不受蛋白质合成抑制剂环己酰亚胺预处理的影响。结果表明，在没有毒蕈碱受体阻断的情况下，大多数副交感神经诱导的脱颗粒可归因于非肾上腺素能、非胆碱能机制的作用。
