Johnston A R, Dutia M B
Department of Physiology, Medical School, Edinburgh, UK.
Neurosci Lett. 1996 Nov 15;219(1):17-20. doi: 10.1016/s0304-3940(96)13152-9.
The timecourse of the appearance and maturation of intrinsic tonic activity in medial vestibular nucleus (MVN) neurones was examined using extracellular single unit recording techniques in slices of the dorsal brainstem prepared from balb/c mice at specific stages during the first postnatal month. In slices from animals at postnatal day 5 (P5), the intrinsic spontaneous discharge rate was low (< 5 impulses/s on average). Over the period P10 to P30 this gradually increased to levels comparable to those of adult MVN cells in vitro. There was a rostro-caudal gradient in the time-course of development of tonic activity, such that cells located rostrally within the MVN developed higher frequencies of tonic discharge earlier than caudally located cells. The opening of the eyes around P14 was associated with a significant increase in the mean discharge rate of caudally located, but not rostrally located, MVN cells.
运用细胞外单单位记录技术，在出生后第一个月特定阶段的balb/c小鼠制备的脑桥背侧脑干切片中，研究内侧前庭核（MVN）神经元内在紧张性活动的出现和成熟的时间进程。在出生后第5天（P5）动物的切片中，内在自发放电率较低（平均<5次冲动/秒）。在P10到P30期间，这一放电率逐渐增加至与体外成年MVN细胞相当的水平。紧张性活动的发育时间进程存在头-尾梯度，使得MVN内靠前位置的细胞比靠后位置的细胞更早出现更高频率的紧张性放电。大约在P14睁眼时，靠后位置而非靠前位置的MVN细胞的平均放电率显著增加。
