Zdichavsky M, Schorpp C, Nickels A, Koch B, Pfreundschuh M, Gause A
Saarland Medical School, Homburg/Saar, Germany.
Rheumatol Int. 1996;16(4):151-7. doi: 10.1007/BF01419728.
We used a double-immunostaining technique to analyze the distribution of bcl-2+ B and T lymphocytes within the synovial membranes (SM) of 13 patients with rheumatic diseases: 11 with rheumatoid arthritis (RA), 1 with ankylosing spondylitis (AS), and 1 with osteoarthritis (OA). A high proportion (up to 50%) of the lymphocytes belonged to the B cell subset. Most of both T and B lymphocytes were positive for the bcl-2 protein. In germinal centers B lymphocytes were also negative for bcl-2 protein expression, comparable to the situation in germinal centers of secondary lymphatic organs. We conclude that bcl-2- B lymphocytes are submitted to antigen selection in the inflamed SMs while bcl-2 protein expression provides survival signals for their persistence in the infiltrates. The expression of bcl-2 may be an important factor in protecting lymphocytes in SM from apoptosis by glucocorticoids, cytostatic drugs, and irradiation.
我们采用双重免疫染色技术分析了13例风湿性疾病患者滑膜中bcl-2阳性的B淋巴细胞和T淋巴细胞的分布情况，其中11例类风湿关节炎（RA）患者、1例强直性脊柱炎（AS）患者和1例骨关节炎（OA）患者。高比例（高达50%）的淋巴细胞属于B细胞亚群。大多数T淋巴细胞和B淋巴细胞的bcl-2蛋白呈阳性。生发中心的B淋巴细胞bcl-2蛋白表达也为阴性，这与二级淋巴器官生发中心的情况类似。我们得出结论，在炎症滑膜中，bcl-2阴性的B淋巴细胞接受抗原选择，而bcl-2蛋白表达为其在浸润中持续存在提供生存信号。bcl-2的表达可能是保护滑膜中淋巴细胞免受糖皮质激素、细胞抑制药物和辐射诱导凋亡的重要因素。
