鸡M2乙酰胆碱受体启动子区域的分离与特性分析：白血病抑制因子和睫状神经营养因子对基因转录的诱导作用
Isolation and characterization of the chicken m2 acetylcholine receptor promoter region: induction of gene transcription by leukemia inhibitory factor and ciliary neurotrophic factor.
作者信息
Rosoff M L, Wei J, Nathanson N M
机构信息
Department of Pharmacology, University of Washington, Seattle 98195-7750, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14889-94. doi: 10.1073/pnas.93.25.14889.
Abstract
We have isolated the promoter region and determined the start sites of transcription for the gene encoding the chicken m2 (cm2) muscarinic acetylcholine receptor. Transfection experiments, using cm2-luciferase reporter gene constructs, demonstrated that a 789-bp genomic fragment was sufficient to drive high level expression in chicken heart primary cultures, while an additional 1.2-kb region was required for maximal expression in mouse septal/ neuroblastoma (SN56) cells. Treatment of SN56 cells with the cytokines ciliary neurotrophic factor and leukemia inhibitory factor increases expression of endogenous muscarinic acetylcholine receptors and results in a 4- to 6-fold induction of cm2 promoter driven luciferase expression. We have mapped a region of the cm2 promoter that is necessary for induction by cytokines.
摘要
我们已经分离出了鸡毒蕈碱型乙酰胆碱受体m2（cm2）编码基因的启动子区域，并确定了其转录起始位点。使用cm2 - 荧光素酶报告基因构建体进行的转染实验表明，一个789 bp的基因组片段足以驱动鸡心脏原代培养物中的高水平表达，而在小鼠中隔/神经母细胞瘤（SN56）细胞中实现最大表达则需要额外的1.2 kb区域。用细胞因子睫状神经营养因子和白血病抑制因子处理SN56细胞，可增加内源性毒蕈碱型乙酰胆碱受体的表达，并导致cm2启动子驱动的荧光素酶表达诱导4至6倍。我们已经绘制出了cm2启动子中细胞因子诱导所必需的区域。
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