支原体对逆转录病毒特异性反应的模拟与预防

Simulation and prevention of retrovirus--specific reactions by mycoplasmas.

作者信息

Lipp M, Koch E, Brandner G, Bredt W

出版信息

Med Microbiol Immunol. 1979 May 15;167(2):127-36. doi: 10.1007/BF02123562.

Abstract

From human mycosis fungoides tumor-derived cell lines, Mycoplasma hyorhinis was isolated. This mycoplasma shared the following characteristics with retroviruses: uptake of 3H-uridine, but not of 3H-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3H-TMP into high molecular weight material in standard reverse transcriptase assays with the template-primer poly (A) . (dT)12. On the other hand, the specific reverse transcriptase reaction of retroviruses with poly(A) . (dT)12 and poly(C) . (dG) approximately 16 was almost completely abolished in the presence of the mycoplasma. Thus, M. hyorhinis may interfere with identification and isolation procedures for retroviruses.

摘要

从人蕈样霉菌病肿瘤衍生的细胞系中分离出了猪鼻支原体。这种支原体与逆转录病毒具有以下共同特征：在细胞培养中摄取³H - 尿苷，但不摄取³H - 胸腺嘧啶；在蔗糖密度为1.16 g/ml处出现条带，经去污剂处理后部分向逆转录病毒核心密度位置（约等于1.24 g/ml）移动；在以模板引物聚（A）·（dT）₁₂进行的标准逆转录酶测定中，³H - TMP掺入高分子量物质中。另一方面，在支原体存在的情况下，逆转录病毒与聚（A）·（dT）₁₂和聚（C）·（dG）约16的特异性逆转录酶反应几乎完全被消除。因此，猪鼻支原体可能会干扰逆转录病毒的鉴定和分离程序。